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quantikine elisa kit  (R&D Systems)


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    Structured Review

    R&D Systems quantikine elisa kit
    Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elisa+kits/Human+M-CSF+Quantikine+ELISA+Kit/pm42821460-65-17-22
    Average 94 stars, based on 68 article reviews
    quantikine elisa kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Exercise alleviates cognitive dysfunction in Alzheimer's disease mice via skeletal muscle-derived extracellular vesicles that enhance plaque clearance by microglia.
    Article Snippet: Exercise confers cognitive benefits in Alzheimer’s disease (AD), yet the underlying mechanisms remain incompletely understood.. Skeletal muscle functions as an endocrine organ that secretes myokines which affect the homeostasis of extra-muscular organs, including the brain.. Here we found that swimming exercise promotes secretion of skeletal muscle-derived extracellular vesicles (SKM-EVs), which are subsequently taken up via pinocytosis by microglia.

    Article Title: Single-cell transcriptomic analysis reveals intra-tumoral heterogeneity and immunotherapy strategies in high-grade serous ovarian cancer
    Article Snippet: Then the harvested cells were detected with FITC Annexin V Apoptosis Detection Kit I (BD Biosciences, 556547) and stained for Annexin V 5 U/test, and cell apoptosis was detected. .. The conditioned medium (CM) of co-cultured CD8 + T cells and CD133 + IFIT1 + or CD133 + IFIT1 - cells were analyzed to measure IFN-γ or GZMB levels using ELISA kits (R&D Systems, QK285, DY2906-05). ..

    Article Title: Gastrodin alleviates neuronal damage in epileptic cell models by targeting P2RY12 to inhibit microglial hyperactivation.
    Article Snippet: Finally, Western blotting was performed using an anti-P2RY12 antibody (Thermo Fisher, USA, RAB02600) and an HRP-labeled goat anti-rabbit secondary antibody (Beyotime, China, A0208) to verify the specific pull-down of the P2RY12 protein. .. ELISA kits (R&D Systems, USA, DTA00D for TNF-α detection; R&D Systems, USA, DLB50 for IL-1β detection) were used to detect the levels of TNF-α and IL-1β. ..

    Article Title: Veronicastrum sibiricum (L.) Pennell extract alleviates inflammation-induced muscle atrophy through NLRP3 inflammasome regulation and mitochondrial function restoration
    Article Snippet: .. Supernatants were acquired, and secretion of the proinflammatory cytokines TNF-α (cat. no. MTA00B; R&D systems, Inc.) and IL-1β was analyzed using ELISA kits (cat. no. DY401; R&D Systems, Inc.) according to the manufacturer's guidelines. .. To acquire lysates from myotubes in vitro , cells were treated with 1% protease and 1% phosphatase inhibitors in RIPA buffer (Biosesang) at room temperature for 30 min. To acquire lysates in vivo , gastrocnemius (GAS) samples were lysed using PRO-PREP TM (Intron Biotechnology, Inc.) at room temperature for 30 min. After Bradford assay quantification of protein concentrations, 30 μg of protein were separated by SDS-PAGE using 10% polyacrylamide gels.

    Article Title: Integrated analysis of age-related microbiome and metabolites reveals youth-associated metabolites in young Korean women's skin.
    Article Snippet: Recent research highlights crucial age-dependent changes in the skin microbiome that impact skin health and the visible signs of aging.. With age, the diversity, composition, and resilience of skin microbial communities shift significantly, often resulting in reduced stability of key commensals such as Cutibacterium acnes and increased abundance of other taxa (Swaney et al. 2025).. These microbial alterations are closely linked to hallmark aging processes, including impaired barrier integrity, increased inflammation, loss of skin elasticity, and wrinkle formation.

    Article Title: Translational toolkit for reproducible, cross-study profiling of human ageing hallmarks in human blood and tissue
    Article Snippet: .. Serum and EDTA plasma concentration of CXCL-9, GDF-15, IL-6 and TNFα, were measured using commercially available ELISA kits (CXCL9; DY392-05, GDF15; DY957, IL-6; DY206, TNF; DY210, R&D Systems, MN, USA) following the manufacturer’s protocol. ..

    Article Title: Prenatal bisphenol A exposure reprograms SF1-lactylation pathways to promote endometriosis susceptibility
    Article Snippet: .. Quantification of IL6, TNFα, and MCP1 in mouse serum or peritoneal fluid was performed using ELISA kits (IL-6: #M6000B, TNF-α: #MTA00B, MCP1: #MJE00B), from R&D Systems, in accordance with the protocols provided by the manufacturer. ..

    Article Title: Plant extract mixture shows anti-inflammatory and barrier-strengthening effects and activates aryl hydrocarbon receptor in a 2D psoriasis model
    Article Snippet: .. TNFα and IL-8 concentrations were determined using commercial ELISA kits (human TNFα or human CXCL8/IL-8 DuoSet ELISA, R&D Systems, Minneapolis, MN, USA) following the manufacturer’s protocol. .. NHEKs were washed with PBS and lysed by scraping them directly from the culture dish in lysis buffer (10 mM Tris pH 8, 1 mM EDTA, 0.5 mM EGTA, 1% Triton-X 100, 0.1% sodium deoxycholate, 0.1% SDS, 140mM NaCl and 1x protease inhibitor cocktail (Roche, Basel, Switzerland)).

    Expressing:

    Article Title: Integrated analysis of age-related microbiome and metabolites reveals youth-associated metabolites in young Korean women's skin.
    Article Snippet: Recent research highlights crucial age-dependent changes in the skin microbiome that impact skin health and the visible signs of aging.. With age, the diversity, composition, and resilience of skin microbial communities shift significantly, often resulting in reduced stability of key commensals such as Cutibacterium acnes and increased abundance of other taxa (Swaney et al. 2025).. These microbial alterations are closely linked to hallmark aging processes, including impaired barrier integrity, increased inflammation, loss of skin elasticity, and wrinkle formation.

    Clinical Proteomics:

    Article Title: Translational toolkit for reproducible, cross-study profiling of human ageing hallmarks in human blood and tissue
    Article Snippet: .. Serum and EDTA plasma concentration of CXCL-9, GDF-15, IL-6 and TNFα, were measured using commercially available ELISA kits (CXCL9; DY392-05, GDF15; DY957, IL-6; DY206, TNF; DY210, R&D Systems, MN, USA) following the manufacturer’s protocol. ..

    Concentration Assay:

    Article Title: Translational toolkit for reproducible, cross-study profiling of human ageing hallmarks in human blood and tissue
    Article Snippet: .. Serum and EDTA plasma concentration of CXCL-9, GDF-15, IL-6 and TNFα, were measured using commercially available ELISA kits (CXCL9; DY392-05, GDF15; DY957, IL-6; DY206, TNF; DY210, R&D Systems, MN, USA) following the manufacturer’s protocol. ..



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    Effect of silibinin administration on A <t>BDNF</t> (ng/ml) and <t>B</t> <t>CaMKII</t> (ng/mg) in sleep-deprived rats with and without consequent 24-h recovery. Data are represented as mean ± SD ( n = 8); group comparisons were performed using one-way ANOVA, followed by Tukey’s multiple-comparisons test. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus the control group; $ P < 0.05, $$ P < 0.01, and $$$ P < 0.001 versus the SD group; # P < 0.05, ## P < 0.01, and ### P < 0.001 versus the SD + R group. SD: sleep-deprived untreated group; SD + R: sleep-deprived untreated group with recovery; SD + Sil: sleep-deprived group treated with silibinin; SD + Sil + R: sleep-deprived group treated with silibinin followed by recovery. BDNF: brain‑derived neurotrophic factor; CaMKII: calcium/calmodulin‑dependent protein kinase II
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    Effect of silibinin administration on A <t>BDNF</t> (ng/ml) and <t>B</t> <t>CaMKII</t> (ng/mg) in sleep-deprived rats with and without consequent 24-h recovery. Data are represented as mean ± SD ( n = 8); group comparisons were performed using one-way ANOVA, followed by Tukey’s multiple-comparisons test. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus the control group; $ P < 0.05, $$ P < 0.01, and $$$ P < 0.001 versus the SD group; # P < 0.05, ## P < 0.01, and ### P < 0.001 versus the SD + R group. SD: sleep-deprived untreated group; SD + R: sleep-deprived untreated group with recovery; SD + Sil: sleep-deprived group treated with silibinin; SD + Sil + R: sleep-deprived group treated with silibinin followed by recovery. BDNF: brain‑derived neurotrophic factor; CaMKII: calcium/calmodulin‑dependent protein kinase II
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    Image Search Results


    BacGuard promotes colon tissue repairment. a) Scheme of microbiota-dependent epithelial repair mechanism orchestrated by BacGuard. b) Short-chain fatty acid (SCFA) profile alterations. n = 6. c) BacGuard-induced probiotic proliferation and d) quantitative results. n = 3. e) Immunofluorescence analysis of ILC3 (ROR γt + CD3 − cells) in colon tissue. f) Flow cytometric analysis of lamina propria lymphocytes (ROR γt + ). n = 3. g) Concentration of IL-22 in MNK-3 cells. n = 3. h) Representative PAS-staining (upper panel) and MUC-2 immunohistochemistry (lower panel) images of colon tissues. n = 5. ns, not significant; ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Dynamic feedback BacGuard anchors microbial metabolism to host symbiosis in real-time ulcerative colitis therapy

    doi: 10.1016/j.bioactmat.2026.05.060

    Figure Lengend Snippet: BacGuard promotes colon tissue repairment. a) Scheme of microbiota-dependent epithelial repair mechanism orchestrated by BacGuard. b) Short-chain fatty acid (SCFA) profile alterations. n = 6. c) BacGuard-induced probiotic proliferation and d) quantitative results. n = 3. e) Immunofluorescence analysis of ILC3 (ROR γt + CD3 − cells) in colon tissue. f) Flow cytometric analysis of lamina propria lymphocytes (ROR γt + ). n = 3. g) Concentration of IL-22 in MNK-3 cells. n = 3. h) Representative PAS-staining (upper panel) and MUC-2 immunohistochemistry (lower panel) images of colon tissues. n = 5. ns, not significant; ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001.

    Article Snippet: For the detection of IL-22 and LPS concentrations, the Mouse IL-22 Precoated ELISA Kit (DAKEWE, China) and Mouse LPS ELISA Kit (JONLNBIO, China) were employed correspondingly.

    Techniques: Immunofluorescence, Concentration Assay, Staining, Immunohistochemistry

    Journal: Advances in Pharmacological and Pharmaceutical Sciences

    Article Title: Evaluation of Biological Effect of Triticum vulgare Extract Compared With Hyaluronic Acid in In Vitro Tissue Models

    doi: 10.1155/adpp/8857215

    Figure Lengend Snippet: Primer sequences used for qRT‐PCR.

    Article Snippet: Supernatants of scratched HaCaT cells (treated and untreated) and supernatants of 3D skin previously treated with LPS and after being scratched were collected after 48 and 72 h, respectively, and analyzed using ELISA for quantification of HBD2 production (Elabscience) according to the manufacturer’s instructions.

    Techniques:

    Effect of silibinin administration on A BDNF (ng/ml) and B CaMKII (ng/mg) in sleep-deprived rats with and without consequent 24-h recovery. Data are represented as mean ± SD ( n = 8); group comparisons were performed using one-way ANOVA, followed by Tukey’s multiple-comparisons test. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus the control group; $ P < 0.05, $$ P < 0.01, and $$$ P < 0.001 versus the SD group; # P < 0.05, ## P < 0.01, and ### P < 0.001 versus the SD + R group. SD: sleep-deprived untreated group; SD + R: sleep-deprived untreated group with recovery; SD + Sil: sleep-deprived group treated with silibinin; SD + Sil + R: sleep-deprived group treated with silibinin followed by recovery. BDNF: brain‑derived neurotrophic factor; CaMKII: calcium/calmodulin‑dependent protein kinase II

    Journal: Molecular Neurobiology

    Article Title: Silibinin Alleviates Cognitive Dysfunction, Biochemical Alterations, and Hippocampal Structural Changes Induced by 5 days of Sleep Deprivation with or Without a Subsequent 24-H Recovery in Rats

    doi: 10.1007/s12035-026-06195-y

    Figure Lengend Snippet: Effect of silibinin administration on A BDNF (ng/ml) and B CaMKII (ng/mg) in sleep-deprived rats with and without consequent 24-h recovery. Data are represented as mean ± SD ( n = 8); group comparisons were performed using one-way ANOVA, followed by Tukey’s multiple-comparisons test. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus the control group; $ P < 0.05, $$ P < 0.01, and $$$ P < 0.001 versus the SD group; # P < 0.05, ## P < 0.01, and ### P < 0.001 versus the SD + R group. SD: sleep-deprived untreated group; SD + R: sleep-deprived untreated group with recovery; SD + Sil: sleep-deprived group treated with silibinin; SD + Sil + R: sleep-deprived group treated with silibinin followed by recovery. BDNF: brain‑derived neurotrophic factor; CaMKII: calcium/calmodulin‑dependent protein kinase II

    Article Snippet: Furthermore, hippocampal tissue homogenates were prepared to determine BDNF (Boster Biological Technology, Pleasanton, CA, USA; Cat. No. EK0308) and CaMKII (BlueGene Biotech, Shanghai, China; Cat. No. E02C0682) using commercially available ELISA kits supplied by Bio-KITS (Cairo, Egypt), according to the manufacturers' instructions.

    Techniques: Control